[CP2K-user] [CP2K:20449] Re: Metadynamics 2 CVs

Marcella Iannuzzi marci.akira at gmail.com
Sun Jul 14 10:41:49 UTC 2024


Hi Leonardo

The input seems OK
Have you got any error message?
Does it work with anyone of the CVs if alone?
Have you tried with a simplified input, for instance without walls, without 
multiple walkers?
Does it work without crashing if you set DO_HILLS to false?
Regards
Marcella

On Friday, July 12, 2024 at 9:56:20 PM UTC+2 leoser... at gmail.com wrote:

> Dear CP2K Developers,
>
> I hope this email finds you well. My name is Leonardo, and I am currently 
> working on simulations involving an endonuclease. I am reaching out to 
> request your assistance with my two collective variable (CV) metadynamics 
> simulation in CP2K.
>
> In my study, I am using two CVs and 10 walkers. The path has already been 
> optimized using string method with the same CVs and 10 replicas. The CVs I 
> am using are:
>
>    1. The distance difference of the O-P bond (nucleophile-electrophile) 
>    and the scissile P-O bond that dissociates.
>    2. The distance between the metal and the leaving group (Mg-O).
>
> However, I am encountering an issue where the simulation stops when the 
> Gaussian is about to spawn. For instance, if I set NT_HILLS to 50, the 
> simulation stops at 50 steps. Interestingly, if I run the metadynamics 
> using only one of the CVs, it works perfectly.
>
> I would appreciate any guidance or suggestions you can provide to resolve 
> this issue. I have attached my input file for your reference.
>
> Thank you for your time and assistance.
>
> Best regards,
>
> ----------
>
> @SET PDB 1.pdb
> @SET ID 1
> @SET STEPS 10000
> @SET WW 0.1
> @SET HILLS 50
> @SET TOP APE1.parm7
>
> &GLOBAL
>   PROJECT APE1
>   PRINT_LEVEL LOW
>   RUN_TYPE MD
> &END GLOBAL
>  
> &FORCE_EVAL
>   METHOD QMMM
>   STRESS_TENSOR ANALYTICAL
>   &DFT
>     CHARGE -1
>     BASIS_SET_FILE_NAME   ./GTH_BASIS_SETS 
>     POTENTIAL_FILE_NAME   ./GTH_POTENTIALS
>     &MGRID   
>       COMMENSURATE
>       CUTOFF 480
>         REL_CUTOFF 50 
>         &END MGRID
>     &QS
>       METHOD GPW
>       EPS_DEFAULT 1.0E-8 
>       EXTRAPOLATION ASPC
>       EXTRAPOLATION_ORDER 3
>     &END QS
>     &SCF
>       MAX_SCF 10
>       EPS_SCF 2.0E-6 
>       SCF_GUESS HISTORY_RESTART
>       &OT 
>         SAFE_DIIS T
>         STEPSIZE 0.15
>         ENERGY_GAP 0.1
>         MINIMIZER DIIS
>         PRECONDITIONER FULL_SINGLE_INVERSE 
>       &END OT
>       &OUTER_SCF
>         EPS_SCF 2.0E-6
>         MAX_SCF 10
>       &END OUTER_SCF
>       &PRINT
>       &RESTART_HISTORY
>         ADD_LAST NUMERIC
>         BACKUP_COPIES 5 # ASPC order + 2
>         &EACH
>         __ROOT__ 1
>         MD 1
>         QS_SCF 0
>         &END EACH
>       &END RESTART_HISTORY
>       &END PRINT
>     &END SCF  
>     &XC
>       &XC_FUNCTIONAL BLYP    
>       &END XC_FUNCTIONAL
>       &XC_GRID
>           XC_DERIV SPLINE2_smooth
>           XC_SMOOTH_RHO NN10
>       &END XC_GRID
>       &VDW_POTENTIAL
>         POTENTIAL_TYPE PAIR_POTENTIAL
>         &PAIR_POTENTIAL
>           PARAMETER_FILE_NAME DFTD3
>           TYPE DFTD3
>           REFERENCE_FUNCTIONAL BLYP
>         &END PAIR_POTENTIAL
>       &END VDW_POTENTIAL
>     &END XC
>     &POISSON
>       POISSON_SOLVER WAVELET
>       PERIODIC NONE
>     &END POISSON
>     &END DFT
>   &MM
>     &FORCEFIELD
>       PARMTYPE AMBER
>       PARM_FILE_NAME ${TOP}
>       &SPLINE
>         EMAX_SPLINE 1.0E8
>         RCUT_NB [angstrom] 10
>       &END SPLINE
>     &END FORCEFIELD
>     &POISSON
>       &EWALD
>         EWALD_TYPE SPME
>         ALPHA .40
>         GMAX 96
>         O_SPLINE 6 
>       &END EWALD
>     &END POISSON
>     &NEIGHBOR_LISTS
>       GEO_CHECK F
>     &END NEIGHBOR_LISTS
>   &END MM
>   &SUBSYS
>     &CELL
>       ABC [angstrom]  90 90 90
>       PERIODIC  XYZ
>     &END CELL
>     &TOPOLOGY
>       CONN_FILE_FORMAT AMBER
>       CONN_FILE_NAME ${TOP}
>       COORD_FILE_FORMAT PDB
>       COORD_FILE_NAME ${PDB}
>     &END TOPOLOGY
>
>     &KIND NA+
>       ELEMENT Na
>     &END KIND
>     &KIND CL-
>       ELEMENT Cl
>     &END KIND
>     &KIND H
>       BASIS_SET DZVP-GTH  
>       POTENTIAL GTH-BLYP-q1
>     &END KIND
>     &KIND O
>       BASIS_SET DZVP-GTH
>       POTENTIAL GTH-BLYP-q6
>     &END KIND
>     &KIND C
>       BASIS_SET DZVP-GTH
>       POTENTIAL GTH-BLYP-q4
>     &END KIND
>     &KIND N
>       BASIS_SET DZVP-GTH
>       POTENTIAL GTH-BLYP-q5
>     &END KIND
>     &KIND P
>       BASIS_SET TZV2P-GTH
>       POTENTIAL GTH-BLYP-q5
>     &END KIND
>     &KIND MG
>       BASIS_SET TZV2P-GTH
>       POTENTIAL GTH-BLYP-q10
>     &END KIND
>
>         
>     &COLVAR
>        &COMBINE_COLVAR
>           
>  &COLVAR
>              &DISTANCE
>                  ATOMS 5827 4693
>              &END DISTANCE
>            &END COLVAR
>           
>          &COLVAR
>              &DISTANCE
>                  ATOMS 4693 4692
>              &END DISTANCE
>            &END COLVAR
>           
>  FUNCTION CV1-CV2
>            VARIABLES CV1 CV2
>            ERROR_LIMIT 1.0E-8
>       
>  &END COMBINE_COLVAR
>     &END COLVAR
>
>     &COLVAR
>      &DISTANCE
>        ATOMS 4692 5694
>      &END DISTANCE
> &END COLVAR
>
>   &END SUBSYS
>
>     &QMMM
>     &CELL
>       ABC 20 20 20
>       PERIODIC XYZ
>     &END CELL
>     &WALLS
>       K 0.06
>       TYPE QUADRATIC
>     &END WALLS
>     E_COUPL GAUSS
>     CENTER SETUP_ONLY
>     NOCOMPATIBILITY T
>     USE_GEEP_LIB 10 
>
>     &QM_KIND O
>       MM_INDEX 390 420 421 855 856 1988 2642 2643 2673 4696 4694 4695 
> 4692 5827 5830 5833 4672 4702 4705  
>     &END QM_KIND
>     &QM_KIND H
>       MM_INDEX 387 388 392 393 417 418 849 850 852 853 1980 1981 1984 
> 1986 1989 1991 1993 2639 2640 2670 2671 2675 2676 4217 4218 4221 4223 4225 
> 4227 4698 4688 4699 5828 5829 5831 5832 5834 5835 4690 4691 4671 4674 4701 
> 4708 4711 4710 4704 4706     
>     &END QM_KIND
>     &QM_KIND C
>       MM_INDEX 386 389 416 419 848 851 854 1979 1982 1983 1985 1987 1990 
> 1992 2638 2641 2669 2672 4216 4219 4222 4226 4697 4687 4670 4673 4689 4700 
> 4707 4709 4703
>     &END QM_KIND
>     &QM_KIND N
>       MM_INDEX 391 2674 4220 4224
>     &END QM_KIND
>     &QM_KIND P
>       MM_INDEX 4693
>     &END QM_KIND
>     &QM_KIND MG
>       MM_INDEX 5694 
>     &END QM_KIND
>         
>     &LINK
>       QM_INDEX            386
>       MM_INDEX            384
>       LINK_TYPE           IMOMM
>     &END LINK
>     
>     &LINK
>       QM_INDEX            416
>       MM_INDEX            414
>       LINK_TYPE           IMOMM
>     &END LINK       
>     
>     &LINK
>       QM_INDEX            848
>       MM_INDEX            846
>       LINK_TYPE           IMOMM
>     &END LINK       
>     
>     &LINK
>       QM_INDEX            1979 
>       MM_INDEX            1977
>       LINK_TYPE           IMOMM
>     &END LINK     
>     
>     &LINK
>       QM_INDEX            2638 
>       MM_INDEX            2636
>       LINK_TYPE           IMOMM
>     &END LINK 
>
>     &LINK
>       QM_INDEX            2669 
>       MM_INDEX            2667
>       LINK_TYPE           IMOMM
>     &END LINK 
>
>     &LINK
>       QM_INDEX            4216 
>       MM_INDEX            4214
>       LINK_TYPE           IMOMM
>     &END LINK 
>
>     &LINK
>       QM_INDEX            4707
>       MM_INDEX            4712
>       LINK_TYPE           IMOMM
>     &END LINK             
>
>     &LINK
>       QM_INDEX            4673
>       MM_INDEX            4675
>       LINK_TYPE           IMOMM
>     &END LINK 
>
>     &LINK
>       QM_INDEX            4670
>       MM_INDEX            4667
>       LINK_TYPE           IMOMM
>     &END LINK 
>     
>   &END QMMM
> &END FORCE_EVAL
>  
> &MOTION
>   &MD
>   ENSEMBLE NVT
>   TIMESTEP [fs] 0.5
>   STEPS       ${STEPS}
>   TEMPERATURE 300
>     &THERMOSTAT
>       TYPE                         NOSE
>       REGION                       GLOBAL
>       &NOSE
>         LENGTH                     3
>         YOSHIDA                    3
>         TIMECON                    50
>         MTS                        2
>       &END NOSE
>     &END THERMOSTAT
>   &END MD 
>   
>     &FREE_ENERGY
>     METHOD METADYN
>     &METADYN
>       DO_HILLS
>       NT_HILLS ${HILLS} 
>       WW [kcalmol] ${WW}
>       
> &METAVAR         
>                         SCALE 0.1
> COLVAR 1
> &WALL
>           POSITION 2
>           TYPE QUADRATIC
>             &QUADRATIC
>             DIRECTION WALL_PLUS
>             K 0.01
>             &END QUADRATIC
>           &END WALL
> &WALL
>           POSITION -2
>           TYPE QUADRATIC
>             &QUADRATIC
>             DIRECTION WALL_MINUS
>             K 0.01
>             &END QUADRATIC
>           &END WALL
>       &END METAVAR
>       
>                         &METAVAR
>                         SCALE 0.1
> COLVAR 2
>                                 &WALL
>                                         POSITION 4.5
>                                         TYPE QUADRATIC
>                                                 &QUADRATIC
>                                                         DIRECTION WALL_PLUS
>                                                         K 0.01
>                                                 &END QUADRATIC
>                                 &END WALL
>                                 &WALL
>                                         POSITION 2.0
>                                         TYPE QUADRATIC
>                                                 &QUADRATIC
>                                                         DIRECTION 
> WALL_MINUS
>                                                         K 0.01
>                                                 &END QUADRATIC
>                                 &END WALL
>                         &END METAVAR
>
> &MULTIPLE_WALKERS
>       NUMBER_OF_WALKERS 10
>               WALKER_COMM_FREQUENCY 1
> WALKER_ID ${ID}
>       &WALKERS_FILE_NAME
>       /home/leo/Documents/APE1/MTD/WALK1/walk1.dat
>       /home/leo/Documents/APE1/MTD/WALK2/walk2.dat
>                                                         
> /home/leo/Documents/APE1/MTD/WALK3/walk3.dat
>                                                         
> /home/leo/Documents/APE1/MTD/WALK4/walk4.dat
>                                                         
> /home/leo/Documents/APE1/MTD/WALK5/walk5.dat
>                                                         
> /home/leo/Documents/APE1/MTD/WALK6/walk6.dat
>                                                         
> /home/leo/Documents/APE1/MTD/WALK7/walk7.dat
>                                                         
> /home/leo/Documents/APE1/MTD/WALK8/walk8.dat
>                                                         
> /home/leo/Documents/APE1/MTD/WALK9/walk9.dat
>                                                         
> /home/leo/Documents/APE1/MTD/WALK10/walk10.dat
> &END WALKERS_FILE_NAME 
>     &END MULTIPLE_WALKERS
>     &PRINT
>         &COLVAR
>             COMMON_ITERATION_LEVELS 4
>           &EACH
>               MD 1
>             &END EACH
>         &END COLVAR
> &END PRINT
>     &END METADYN
>   &END FREE_ENERGY
>   
>   &PRINT
>     &TRAJECTORY                                 
>       FORMAT DCD                              
>       &EACH                                    
>         MD 4
>       &END EACH
>     &END TRAJECTORY
>     &RESTART_HISTORY                            
>       &EACH                                     
>         MD 50
>       &END EACH
>     &END RESTART_HISTORY
>     &END PRINT
> &END MOTION
>

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